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French Press Cell Lysis: How Does It Compare to Homogenization?

The French pressure cell press, or French press, is a piece of equipment used in laboratories to disrupt cell walls and cell membranes. The French press consists of a hydraulic pump that drives a piston. The piston forces the liquid sample through a tiny valve under high pressure. As the sample passes through the valve, the cells experience shear stress, resulting in cellular disruption...

Why Scalability Matters for Particle Size Reduction Equipment

Process manufacturing is the branch of manufacturing associated with ingredients or raw materials, formulas, and manufacturing recipes. This kind of manufacturing is common in the food, beverage, chemical, pharmaceutical, consumer packaged goods, and biotechnology industries. Scalability is a crucial element in process manufacturing, as production processes must be proportionally adjustable to adapt to production needs.

Probiotics

The intestinal tract is home to trillions of bacteria that can have a huge impact on overall pet health. An imbalance in bacterial populations can affect the body’s immune system, lead to digestive disorders, inflammation of the intestines or diarrhea. The gut microbiota can even affect brain development and behavior. Probiotics are live bacteria that can help
to shift gut microbiota toward more beneficial bacterial
species, helping maintain an optimal balance

Cell Rupture: Detergents vs Traditional Physical Methods

Detergents (surfactants) are used in cell lysis solutions because they disrupt the distinct interface between hydrophobic and hydrophilic systems. They help to solubilize membrane proteins and lipids, thereby causing the cell to lyse and release its contents. Detergents are comprised of a polar hydrophilic head group and a nonpolar hydrophobic tail. They are categorized by the nature of the head group as either ionic, nonionic or zwitterionic.

Types of Emulsifiers & How High Pressure Homogenization Creates Stable Emulsions

An emulsifier (or an emulgent) is a substance that essentially helps in the formation and stabilization of an emulsion. Emulsifiers are surface-active agents that act as the interface between the two immiscible liquids, like oil and water. Emulsifier molecules have a hydrophilic end that forms chemical bonds with water but not with oils; and a hydrophobic end that forms chemical bonds with oils but not with water.

How Homogenization Benefits Emulsions in the Food Industry

An emulsion is a mixture of two immiscible liquids (like oil and water), where small droplets of one liquid are dispersed throughout the second liquid. Emulsions can be classified as follows: Oil in water emulsion – where oil droplets are dispersed in the water phase e.g. homogenized milk or a “creamy” style salad dressing. Water in oil emulsion – where water droplets are dispersed in an oil phase e.g. margarine.
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5 Reasons Why You Need a High Pressure Homogenizer in Your Biotech Research Laboratory

Biotechnology is healing, fueling and feeding the world. Groundbreaking innovations in the fields of medicine, energy and food production are changing the way that we live. In the laboratory, biotechnology harnesses cellular and biomolecular processes to develop technologies and products that help improve our lives and the health of our planet.

DeBEE Laboratory High Pressure Homogenizers are ideal for rupturing a variety of different cell types, including E. coli, yeast, mammalian tissue, bacteria...

Chemical Methods of Cell Disruption

Cell disruption, or cell lysis, is achieved when the cell wall or membrane is ruptured, releasing the contents of the cell. Cell disruption is the first step in many biotechnology applications. This sensitive process needs to be controlled as much as possible; and choosing the correct cell lysis method is essential for preserving the desired intracellular contents.

Cell disruption can be achieved in several ways, including mechanical, enzymatic or chemical lysis.